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[ROOT]NachoRoot - supporting all firmware jan 3rd 2012
This is the latest root method, support all firmware so no need to downgrade firmware.
[Exclusive] 'NachoRoot' Brings Root Access To The Transformer Prime, Even With The Newest Firmware
You need to have ADB setup.
Good tutorial here
[GUIDE] Getting ADB Setup; An Idiots' proof guide on getting ADB working for Rooting! - xda-developers