Yes you would have to unroot to be able to get the OTA, but why would you want to do that. You won't see any advantage of being on unrooted GB vs GB rom. I would stay rooted unless you need to return your phone. Are you currently running a BG BAMF rom?
@bigcanoe, I wouldn't want to unroot since you will also gain all that bloatware. I myself am not rooted yet, though looking to hop on very soon.
@mslarryjr, is it recommended to backup my device before going from froyo to OTA 2.3?
seems like many people are just updating without worrying. I'd like to go ahead and hit the OTA, but i'm also a little of a cautious type.
You shouldn't lose any data by using the OTA but I would still backup anything you think is important just to be safe. I don't have any experience using the OTA since I'm rooted so I just going off what other users have said.