Auto rotate stopped working Samsung Galaxy S4.

Welcome to Android Central! Are you talking about only the homescreen, or in all other apps as well? Does it persist after a reboot? How about after wiping the cache partition? http://support.t-mobile.com/docs/DOC-5744

You can check if the orientation sensors are working correctly with an app like CPU-Z.
 
If you rooted and deleted Samsung Hub you will need to upload a file to fix. I am not yet able to upload a link to the information, but perhaps someone else can if that's your issue...
 

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